erlenmeyer shaker flask Search Results


cell culture flasks  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology cell culture flasks
Cell Culture Flasks, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/erlenmeyer+shaker+flask/Flasks/10__3390_slash_molecules25092257-426-0-9
Average 94 stars, based on 1 article reviews
cell culture flasks - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier
disposable erlenmeyer 125-ml plastic shaker flasks  (Corning Life Sciences)
90
Corning Life Sciences disposable erlenmeyer 125-ml plastic shaker flasks
Disposable Erlenmeyer 125 Ml Plastic Shaker Flasks, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/erlenmeyer+shaker+flask/disposable+erlenmeyer+125+ml+plastic+shaker+flasks/pm24510545-680-124-133
Average 90 stars, based on 1 article reviews
disposable erlenmeyer 125-ml plastic shaker flasks - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
shaker flasks erlenmeyer culture flasks with a 2 μm vent cap and without baffles  (Corning Life Sciences)
90
Corning Life Sciences shaker flasks erlenmeyer culture flasks with a 2 μm vent cap and without baffles
Shaker Flasks Erlenmeyer Culture Flasks With A 2 μm Vent Cap And Without Baffles, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/erlenmeyer+shaker+flask/shaker+flasks+erlenmeyer+culture+flasks+with+a+2+%CE%BCm+vent+cap+and+without+baffles/pmc05570375-67-5-24
Average 90 stars, based on 1 article reviews
shaker flasks erlenmeyer culture flasks with a 2 μm vent cap and without baffles - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
vented corning erlenmeyer shaker flasks  (Corning Life Sciences)
90
Corning Life Sciences vented corning erlenmeyer shaker flasks
Optimisation of culture conditions and r CLU expression in <t>MEXi</t> <t>293E</t> cells. ( A ) and ( B ) Data shown for MEXi293E cells transfected with pEGFP-N1; means + /− SEM (n = 3) are plotted. In some cases the error bars are too small to be visible. ( A ) Left Panel: Viable cell density over 5 days post transfection (DPT). Right Panel: Percent cell viability over 5 DPT. ( B ) Percent of cells transiently expressing GFP measured for 5 DPT. ( C ) Transfection efficiency of cells overexpressing r CLU-αC-ST and r CLU-CT were measured by immunostaining of fixed, permeabilised cells on day 5 post-transfection. Untransfected (UN) cells were used as control. To detect r CLU-αC-ST, cells were stained with anti-strep tag antibody (Anti-ST Ab) or DNP9 (an isotype-matched antibody of irrelevant specificity) followed by goat anti-mouse IgG-CF488. r CLU-CT was detected using biotinylated anti-C tag conjugate ( b -CT-Ab) followed by streptavidin-CF488 (SA-488); the negative control in this case was cells incubated with SA-488 alone. ( D ) Densitometric analysis of Western blot with culture supernatant from cells overexpressing r CLU-αC-ST harvested on day 5 DPT and probed with streptactin-HRP conjugate. Cells were either supplemented with tryptone N1 (TN1) or sodium butyrate (NaB) or untreated (UN). Means + /− SEM (n = 3) are plotted; statistically significant differences are indicated by * (Oneway ANOVA, p < 0.001; ns non-significant). The results shown are each representative of two independent experiments.
Vented Corning Erlenmeyer Shaker Flasks, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/erlenmeyer+shaker+flask/vented+corning+erlenmeyer+shaker+flasks/pmc07455699-196-4-12
Average 90 stars, based on 1 article reviews
vented corning erlenmeyer shaker flasks - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
tc shaker flasks, erlenmeyer  (Genesee Scientific)
N/A
TC Shaker Flasks, Erlenmeyer, 125mlPolycarbonate, Flat, Std Cap
   Buy from Supplier

Image Search Results


Optimisation of culture conditions and r CLU expression in MEXi 293E cells. ( A ) and ( B ) Data shown for MEXi293E cells transfected with pEGFP-N1; means + /− SEM (n = 3) are plotted. In some cases the error bars are too small to be visible. ( A ) Left Panel: Viable cell density over 5 days post transfection (DPT). Right Panel: Percent cell viability over 5 DPT. ( B ) Percent of cells transiently expressing GFP measured for 5 DPT. ( C ) Transfection efficiency of cells overexpressing r CLU-αC-ST and r CLU-CT were measured by immunostaining of fixed, permeabilised cells on day 5 post-transfection. Untransfected (UN) cells were used as control. To detect r CLU-αC-ST, cells were stained with anti-strep tag antibody (Anti-ST Ab) or DNP9 (an isotype-matched antibody of irrelevant specificity) followed by goat anti-mouse IgG-CF488. r CLU-CT was detected using biotinylated anti-C tag conjugate ( b -CT-Ab) followed by streptavidin-CF488 (SA-488); the negative control in this case was cells incubated with SA-488 alone. ( D ) Densitometric analysis of Western blot with culture supernatant from cells overexpressing r CLU-αC-ST harvested on day 5 DPT and probed with streptactin-HRP conjugate. Cells were either supplemented with tryptone N1 (TN1) or sodium butyrate (NaB) or untreated (UN). Means + /− SEM (n = 3) are plotted; statistically significant differences are indicated by * (Oneway ANOVA, p < 0.001; ns non-significant). The results shown are each representative of two independent experiments.

Journal: Scientific Reports

Article Title: Rapid high-yield expression and purification of fully post-translationally modified recombinant clusterin and mutants

doi: 10.1038/s41598-020-70990-3

Figure Lengend Snippet: Optimisation of culture conditions and r CLU expression in MEXi 293E cells. ( A ) and ( B ) Data shown for MEXi293E cells transfected with pEGFP-N1; means + /− SEM (n = 3) are plotted. In some cases the error bars are too small to be visible. ( A ) Left Panel: Viable cell density over 5 days post transfection (DPT). Right Panel: Percent cell viability over 5 DPT. ( B ) Percent of cells transiently expressing GFP measured for 5 DPT. ( C ) Transfection efficiency of cells overexpressing r CLU-αC-ST and r CLU-CT were measured by immunostaining of fixed, permeabilised cells on day 5 post-transfection. Untransfected (UN) cells were used as control. To detect r CLU-αC-ST, cells were stained with anti-strep tag antibody (Anti-ST Ab) or DNP9 (an isotype-matched antibody of irrelevant specificity) followed by goat anti-mouse IgG-CF488. r CLU-CT was detected using biotinylated anti-C tag conjugate ( b -CT-Ab) followed by streptavidin-CF488 (SA-488); the negative control in this case was cells incubated with SA-488 alone. ( D ) Densitometric analysis of Western blot with culture supernatant from cells overexpressing r CLU-αC-ST harvested on day 5 DPT and probed with streptactin-HRP conjugate. Cells were either supplemented with tryptone N1 (TN1) or sodium butyrate (NaB) or untreated (UN). Means + /− SEM (n = 3) are plotted; statistically significant differences are indicated by * (Oneway ANOVA, p < 0.001; ns non-significant). The results shown are each representative of two independent experiments.

Article Snippet: In preparation for transfection, MEXi 293E were grown in 250 ml vented Corning Erlenmeyer shaker flasks (Sigma Aldrich, Australia) to a cell density of 2–3 × 10 6 cells/ml, and a cell viability of at least 98%.

Techniques: Expressing, Transfection, Immunostaining, Control, Staining, Strep-tag, Negative Control, Incubation, Western Blot